Question #278e0

2 Answers
Feb 18, 2015

You would need to add 794 muL of the "32%" solution.

So, you know that your final solution is "40%" ethanol, which means you can use its volum to determine exactly how much ethanol it should contain

"%40" = V_("ethanol")/V_("solution") * 100 => V_("ethanol") = (40 * V_("solution"))/100

V_("ethanol") = (40 * 900 muL)/100 = 360 muL

Now, this amount of ethanol must come from two sources: the absolute ethanol - I'll label this V_("100") - and from the "32%" solution - I"ll label this V_("solution 32"). You can therefore set up two equations

V_("ethanol") = V_("100") + V_("ethanol 32") (1), and

V_("solution") = V_("100") + V_("solution 32") (2)

Now, you can express the volume of "32%" solution like this

"%32" = V_("ethanol 32")/V_("solution 32") * 100 => V_("solution 32") = V_("ethanol 32") * 100/32

Plug this into equation (2) and you'll get

V_("solution") = V_("100") + V_("ethanol 32") * 100/32

So,

360 = V_("100") + V_("ethanol 32")
900 = V_("100") + V_("ethanol 32") * 100/32

You have V_("100") = 360 - V_("ethanol 32"), and

900 = 360 - V_("ethanol 32") + V_("ethanol 32") * 100/32

Solving this will get you V_("ethanol 32") = 540/2.215 = 254.1 muL

This value represents the amount of ethanol you must get from the "32%" solution, which implies that the total volume for that solution must be

V_("solution 32") = V_("ethanol 32") * 100/32 = 254.1 * 100/32 = 794 muL

If you round this value to one sig fig, the answer will be

V_("solution 32") = 800 muL

Feb 18, 2015

The student should use 790 µL of 32 % ethanol.

Let x = the volume of 100 % ethanol and y = the volume of 32 % ethanol.

Then we can re-write the problem as

"900 µL" × 40 % = x" µL × 100 %" + y" µL × 32 %"

Cancelling units, we get

(1) 36 000 = 100x + 32y

We also know that the total volume must be 900 µL.

(2) x + y = 900

We have two equations in two unknowns.

Solve (2) for x.

(3) x = 900 – y

Substitute (3) into (1).

36 000 = 100(900 – y) + 32y= 90 000 – 100 y + 32 y = 90 000 - 68y

68y = 90 000 -36 000 = 54 000

y = 790

So you would use 790 µL of 32 % ethanol.

Note: The answer can have only 2 significant figures, because that is all you gave for the
32 % ethanol. If you need more precision, you will have to recalculate.