At which stage in mitosis do you first see the sister chromatids becoming chromosomes? Biology The Eukaryotic Cell Mitosis 1 Answer Mandira P. Mar 9, 2017 () Sister chromatids of a chromosome separate and start to travel towards opposite poles during anaphase of mitosis. Sister chromatids become individual chromosome as soon as they separate. Hence answer will be ANAPHASE . Answer link Related questions Why is mitosis necessary for living organisms? How is metaphase distinguished? How do cytokinesis and telophase differ? How do chromosomes divide during anaphase? How can mitosis be a form of asexual reproduction? How does mitosis conserve the chromosome number? How are mitosis and meiosis different? What is anaphase? What is mitosis? What are kinetochores made of? See all questions in Mitosis Impact of this question 18268 views around the world You can reuse this answer Creative Commons License