Benedicts reagent is an indicator for the presence of what in a solution?

1 Answer
Mar 11, 2018

Reducing sugar and reducing sugar like substances like creatinine,uric acid,homogentisic acid etc.

Explanation:

Benedict's reagent conatins, CuS0_4,Na-carbonate and Na-citrate

In presence of alkaline media created due to Na-carbonate reducing sugar undergoes ene-diol transformation as a result of this Cu^(+2) in CuSO_4 (blue) gets reduced to Cu^+ of Cu_2O (red).

Na-citrate causes slow release of Cu^(+2) from CuSO_4

So,change in colour of the solution is indicated as a positive test.

Now,this change in color proceeds through a steps of color changes,which corresponds roughly to certain amount of reducing sugar present.

Blue - 0%

Green -0.5 g %

Yellow - 1 g%

Oran g e - 1.5 g%

Red - 2 g%

Brick Red - _-^> 2.5 g %

So,Benedict's test is also called Semiquantitative test.